{"id":15,"date":"2013-08-22T15:57:41","date_gmt":"2013-08-22T15:57:41","guid":{"rendered":"http:\/\/snrp.uccaribe.net\/?page_id=15"},"modified":"2013-10-22T10:28:11","modified_gmt":"2013-10-22T14:28:11","slug":"project-3","status":"publish","type":"page","link":"https:\/\/snrp.uccaribe.net\/?page_id=15","title":{"rendered":"Activity-dependent Regulation of Synaptic Vesicle Abundance"},"content":{"rendered":"<h1><b>P.I. Maria Bykhovskaia<br \/>\n<\/b><b>Collaborator Dr. J. Troy Littleton, Massachusetts Institute of Technology<\/b><\/h1>\n<p style=\"text-align: left;\" align=\"center\"><a href=\"http:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/09\/P3.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-133\" alt=\"P3\" src=\"http:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/09\/P3.jpg\" width=\"870\" height=\"422\" srcset=\"https:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/09\/P3.jpg 870w, https:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/09\/P3-300x145.jpg 300w, https:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/09\/P3-500x242.jpg 500w\" sizes=\"(max-width: 870px) 100vw, 870px\" \/><\/a><\/p>\n<blockquote>\n<p style=\"text-align: left;\" align=\"center\">Synaptic vesicle cycling is highly dynamic and plastic, and subtle disruptions in its regulation may contribute to severe neurological disorders, including schizophrenia, epilepsy, Huntington\u2019s and Parkinson\u2019s Disease. Although a number of molecular determinants of vesicle cycling at synaptic terminals have been identified, our understanding of how vesicle cycling contributes to plasticity remains sparse. Using the <i>Drosophila<\/i> neuromuscular junction (NMJ), the lab of Dr. Bykhovskaia has discovered a pathway of presynaptic enhancement that may enable a nerve terminal to sustain and enhance its activity upon intense stimulation. Specifically, it was found that vesicle abundance in synaptic boutons is regulated by activity, and that intense stimulation increases vesicle number in nerve terminals. Importantly, these results suggest a functional implication for this form of plasticity: enhanced activity upon a subsequent intense stimulation.<b><i>\u00a0 <\/i><\/b>This project employs a combination of molecular biology and genetics, live confocal imaging, and electron microscopy to unravel the mechanisms by which the terminal regulates its vesicle numbers in response to activity. Further, the project will test whether the phenomenon observed at the <i>Drosophila<\/i> glutamatergic synapse could be also detected in the mammalian central or peripheral synapses.<\/p>\n<\/blockquote>\n<p style=\"text-align: left;\" align=\"center\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-130\" style=\"color: #333333; font-style: normal;\" alt=\"P3-1\" src=\"http:\/\/snrp.uccaribe.net\/wp-content\/uploads\/2013\/08\/P3-1.gif\" width=\"757\" height=\"240\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>P.I. Maria Bykhovskaia Collaborator Dr. J. Troy Littleton, Massachusetts Institute of Technology Synaptic vesicle cycling is highly dynamic and plastic, and subtle disruptions in its regulation may contribute to severe neurological disorders, including schizophrenia, epilepsy, Huntington\u2019s and Parkinson\u2019s Disease. Although a number of molecular determinants of vesicle cycling at synaptic terminals have been identified, our &hellip; <\/p>\n<p><a class=\"more-link btn\" href=\"https:\/\/snrp.uccaribe.net\/?page_id=15\">Continue reading<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-onecolumn.php","meta":[],"_links":{"self":[{"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/pages\/15"}],"collection":[{"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15"}],"version-history":[{"count":6,"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":278,"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=\/wp\/v2\/pages\/15\/revisions\/278"}],"wp:attachment":[{"href":"https:\/\/snrp.uccaribe.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}